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Protein can cause a spike in Insulin, but not aspartame[6]. Furthermore, consuming aspartame with or without carbohydrates resulted in aspartame not contributing to an insulin spike[7].

Diabetics were found to have no spike in insulin after ingesting nonnutritive sweeteners[8]. Swishing a solution in the mouth had no effect[9]

About the only study suggesting sweeteners could spike insulin was found in vitro rat pancreatic cells when coupled with glucose